SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. The structural modifications provide:
Half life of 220 minutes at 50°C for the highest cDNA yields (Figure 1)
Reduced RNase H activity for more full-length cDNA
Full activity at 50°C for increased specificity with GSP
Ability to increase RT units without inhibiting subsequent PCR
注：所有的逆轉(zhuǎn)錄酶都是在37度進(jìn)行反應(yīng),超過這個(gè)溫度就失活。而這個(gè)失活溫度時(shí)55度，可以在50度進(jìn)行反應(yīng)。這樣可以獲得更長的片段。
