熒光光纖氧氣測(cè)量技術(shù)具有高精確度、高可靠性、響應(yīng)時(shí)間短、適用于氣相和液相等優(yōu)勢(shì)，因此隨著技術(shù)的問(wèn)世，精確、高通量測(cè)量微小生物的呼吸和評(píng)估其能量代謝成為可能。高通量呼吸測(cè)量系統(tǒng)基于熒光光纖氧氣測(cè)量技術(shù)，能夠?qū)?strong>斑馬魚的胚胎及幼魚進(jìn)行測(cè)量，測(cè)定其耗氧量，進(jìn)而評(píng)估其代謝水平。系統(tǒng)在生物醫(yī)學(xué)、實(shí)驗(yàn)生物學(xué)、污染生態(tài)學(xué)與環(huán)境毒理學(xué)、環(huán)境科學(xué)、氣候變化研究等領(lǐng)域具有越來(lái)越重要的應(yīng)用價(jià)值。
左：斑馬魚微型呼吸室；右：斑馬魚高通量呼吸代謝測(cè)量系統(tǒng)測(cè)量原理
系統(tǒng)由內(nèi)置熒光光纖氧氣傳感器的微型呼吸室、氧氣測(cè)量主機(jī)及數(shù)據(jù)采集分析軟件組成，可對(duì)96個(gè)通道的樣品進(jìn)行同步測(cè)量。
功能特點(diǎn)
· 氧氣測(cè)量高精度、高可靠性、低功耗、低交叉敏感性、快速響應(yīng)時(shí)間
· 輕松校準(zhǔn)
· 非侵入性和非破壞性測(cè)量
· 緊湊設(shè)計(jì)，適用于溫控培養(yǎng)箱和/或搖床
技術(shù)參數(shù)
1. 檢測(cè)技術(shù)：光纖氧傳感器技術(shù)。
2. 適用場(chǎng)景：原位檢測(cè)，可在培養(yǎng)箱里或搖床上使用，便于溫度控制。
3. 呼吸室：透明聚苯乙烯材質(zhì)，支持預(yù)消毒處理，可重復(fù)使用。
4. 氧氣測(cè)量主機(jī)：?jiǎn)蝹€(gè)重670 g，162 x 102 x 32 mm
5. 主機(jī)內(nèi)置溫度傳感器：0-50°C，分辨率0.012°C，精度±0.5°C
6. 主機(jī)內(nèi)置壓強(qiáng)傳感器：300-1100mbar，分辨率0.11mbar，精度±6mbar
7. 蕞大采樣頻率：?jiǎn)瓮ǖ?span id="rofelhy" class="highlight">激活時(shí)可達(dá)10-20次每秒
8. 氧氣測(cè)量精度：±0.1% O2@1% O2或±0.05 mg/L@0.44 mg/L
9. 氧氣測(cè)量分辨率：0.01% O2@1% O2或0.005 mg/L@0.44 mg/L
10. 電源：5VDC，USB供電
11. 響應(yīng)時(shí)間＜30s
12. 通道數(shù)：96
13. 系統(tǒng)適配其他魚類的胚胎及幼魚
14. 可選配斑馬魚成魚的靜態(tài)及動(dòng)態(tài)呼吸測(cè)量系統(tǒng)
左圖：環(huán)境污染物6PPD和6PPD醌對(duì)斑馬魚幼魚呼吸的影響(Varshney et al., 2022)；
右圖：小丑魚幼魚在不同溫度下的代謝率(Moore et al., 2023)
參考文獻(xiàn)
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