Recombinant Human ACE-2 (C419)
產(chǎn)品說明(Description)
Recombinant Human Angiotensin-Converting Enzyme 2 is produced by our Mammalian expression system and the target gene encoding Gln18-Ser740 is expressed with a 6His tag at the C-terminus.
Accession #:Q9BYF1
Known as: Angiotensin-Converting Enzyme 2; ACE-Related Carboxypeptidase; Angiotensin-Converting Enzyme Homolog; ACEH; Metalloprotease MPROT15; ACE2
制劑(Formulation)
Supplied as a 0.2 μm filtered solution of 20mMTris，300mMNacl,1mMZncl2,10%Glycerol，PH7.4.
質量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity: | |
Immobilized Human ACE-2-His(Cat#C419) at 2μg/ml (100 μl/well)can bind 2019-nCoV S Protein RBD (N501Y, C-Fc)(Cat#DRA121). The ED50 of 2019-nCoV S Protein RBD (N501Y, C-Fc)(Cat#DRA121)is 3.57 ng/ml. | Immobilized Human ACE-2-His(Cat#C419)at 10μg/ml (100μl/well) can bind 2019-nCoV S Protein RBD-mFc(Cat#DRA32). The ED50 of 2019-nCoV S Protein RBD-mFc(Cat#DRA32) is 19.90 ng/ml. |
Bioactivity-BLI: | Bioactivity-Elisa: |
Loaded 2019-nCoV S Protein RBD-mFc(Cat#DRA32) on AMC Biosensor, can bind Human ACE-2-His(Cat#C419) with an affinity constant of 2.06 nM as determined in BLI assay. | Immobilized Human ACE-2-His(Cat#C419) at 5μg/ml (100 μl/well)can bind 2019-nCoV S Protein RBD-Fc(Cat#DRA126). The ED50 of 2019-nCoV S Protein RBD-Fc(Cat#DRA126) is 3.8 ng/ml. |
運輸(Shipping)
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
保存(Storage)
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
背景(Background)
Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don’t inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.
電泳(SDS-PAGE)
參考文獻(Reference)
Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in environmental specimens via surface enhanced Raman scattering (SERS)[J]. Dayi Zhang. et al. medRxiv.2020.
Isolation of a human monoclonal antibody specific for the receptor binding domain of SARS-CoV-2 using a competitive phage biopanning strategy[J]. Xin Zeng.et al. Antibody Therapeutics.2020.
Emergence of RBD mutations from circulating SARS-CoV-2 strains with enhanced structural stability and higher human ACE2 receptor affinity of the spike protein[J]. Junxian Ou. et al. bioRxiv.2020.
FOR RESEARCH USE ONLY
