ADCC and ADCP reporter T-cell lines
Intracellular signaling in Jurkat?-Lucia NFAT-CD16 (or CD32) cells
INVIVOGEN ALSO OFFERS:
? Clinically relevant antibodies in various Ig isotypes
? Raji-target cells expressing a choice of molecules of interest
InvivoGen offers a series of Jurkat-Lucia? NFATcell lines, specifically designed to assess the potency of specific immunoglobulin for ADCC(antibody-dependent cellular cytotoxicity) and ADCP(antibody-dependent cell-mediated phagocytosis).
– Jurkat-Lucia? NFAT-CD16 cells for ADCC reporter assays– Jurkat-Lucia? NFAT-CD32 cells for ADCP reporter assays
These cells derive from the human T-lymphocyte Jurkat cell line and stably express CD16 or CD32, two Fc-gamma receptors (FcγR) for the constant region of immunoglobulins. ADCC and ADCP outcomes depend on the preferential affinity of each antibody (Ab) for one FcγR. The Ab-FcγR interaction is regulated by the Ab isotype and glycosylation. Jurkat cells naturally express a functional NFAT (nuclear factor of activated T cells) transcription factor, which is involved in the early signaling events of ADCC and ADCP [1, 2].
Jurkat-Lucia? NFAT-CD16and Jurkat-Lucia? NFAT-CD32 cells have been designed aseffector reporter cellsfor InvivoGen’s ADCCandADCPassays, respectively.These cells stably express the Lucia luciferase reportergene under the control of an ISG54 minimal promoter fused to six NFAT response elements. ADCC or ADCP induction is measured as a bioluminescent signal produced by the Lucia luciferase upon the addition of the appropriate detection reagent QUANTI-Luc?.
Key Features:
Endogenous NFAT expression
Stable CD16A(FcgRIIIA; V158 allotype [3]) expression
Stable CD32A(FcgRIIA; H131 allotype [4])expression
Readily assessable Lucia luciferase reporter activity for NFAT activation
Resistance to Blasticidin and Zeocin?
Applications:
