Description
The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, raphe nuclei and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/20,000-1/40,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 25 ug of serotonin/BSA.
Cross reactivity of Serotonin antisera was examined. With 5μg, 10μg and 25μg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.
Photo Description:Low magnification IHC image of neurons staining for the 5-HT rabbit antibody in the raphe nucleus of the rat brainstem (above), and corresponding high magnification image of the raphe nucleus (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:25000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity / Volume: 100 μL
State: Lyophilized Whole Serum
Availability: In Stock
Reacts With: Abalone (Haliotis Kamtschatkana) , Amphibian, Annelid Mushroom, Annelida (Segmented Worm), Ant, Ant (Pheidole Dentata), Antalis Entalis Scaphopoda, Aplysia (Sea Slug), Aplysia Californica (Sea Slug), Aplysia Punctata (Sea Slug), Arachnocampa (Gnat), Archidoris Pseudoargus (Sea Slug), Asterina Pectinifera (Starfish ), Astyanax Mexicanus (Cave Fish), Axolotl (Salamander), Bacteria, Beetle, Bird, Brachiopods, Bryozoans (Polyzoa), Buffalo, Bufo Marinus (Toad), Bug, Bumblebee, Butterfly, Butterfly (Monarch), Canine, Cat, Chick, Chicken, Chiton, Chrysoperla Carnea Green Lacewing , Clam, Clione Limacina Pteropod (Fruit Bat), Cockroach, Cod, Crab, Crayfish, Cricket, Crustaceans, Cupiennius Salei (Spider), Desert Locust, Dog, Dogfish (Shark), Dove, Drosophila (Fly), Drosophila Melanogaster (Fly), Feather Star, Ferret, Fish, Fish (Salmon), Flounder, Fly, Frog, Frog (Xenopus Laevis), Fruit Fly, Gastropod (Slug), Gastropod (Slug), Gerbil, Glowworm, Goldfish, Grasshopper, Guinea Pig, Hamster, Hedgehog, Helisoma Trivolvis (Snail), Hen, Hermit Crab, Honeybee, Human, Idiosepius Notoides (Squid), Ilyanassa Obsoleta (Sea Snail), Insect, Kitten (Cat), Lamprey, Leech, Lizard, Lobster, Lobster (Cycliophora), Lobster Larva, Locust, Lythrypnus Dalli (Fish), Macracantha (Cephalocarida), Manduca Sexta (Moth), Marmoset (Monkey), Mercenaria (Clam), Mexican salamander (Ambystoma Mexicanum) , Mollusca, Mollusk, Monkey, Monkey (Macaque), Mosquito, Mosquito (Aedes Aegypti), Moth, Moth (Manduca Sexta), Mouse, Mudpuppy, Mussel, Newt, Nudibanch, Nudibranch Mollusc, Nudipleura, Octopus, Onychophora (Velvet Worm), Ovine (Sheep), Parhedyle Cryptophthalma (Sea Slug), Phoronis Pallida (Phoronida), Phyllodoce Maculata (Phyllodocidae), Phyllodoce Maculata (Polychaeta), Pig, Pigeon, Pilidium Larvae, Platynereis Dumerilii (Ragworm), Platypus, Pleurobranchaea Californica (Sea Slug), Pleurobranchaea Japonica (Sea Slug), Pleurodeles (Newt), Prawn, Praying Mantis, Ptychodera Flava (Worm), Quail, Rabbit, Raja Erinacea (Skate), Ram (Sheep), Rana Perezi (Frog), Rat, Red Abalone, Red Abalone, Renilla Koellikeri (Cnidarian ), Rhodnius Prolixus (Bug), Rhodnius Prolixus (Parasite), Salamander, Sand Dollar, Schistocerca Gregaria (Locust), Scorpaena Porcus (Fish), Scorpion, Scyliorhinus Canicula (Dogshark), Sea Bass (Fish), Schistocerca Gregaria (Locust), Scorpaena Porcus (Fish), Scorpion, Scyliorhinus Canicula (Dogshark), Sea Bass (Fish), Sea Cucumber, Sea Lamprey, Sea Lily, Sea Scallop, Sea Slug, Sea Spider, Sea Star, Sea Urchin, Seahorse, Shark, Sheep, Shrew, Skate, Snail, Sparus Aurata (Fish), Spisula (Clam), Springtails, Squirrel Monkey, Sting Ray, Stomatopod Crustacean, Sturgon, Tapeworm, Tectura Scutum (Mollusk), Tick, Tigriopus Californicus (Copepod), Toad, Tragulus Javanicus (Mouse Deer), Tritonia Diomedea (Sea Slug), Trout, Turkey, Turtle, Worm, Worm (Digenea) , Worm (Diphyllobothrium Dentriticum) , Worm (Dugesia Tigrina) , Worm (Dugesia Tigrina), Worm (Phyllodoce Groenlandica), Worm (Polychaete Capitella), Zebra Finch (Bird), Zebrafish
