DNA Clean & Concentrator-100
| Cat # | Name | Size |
|---|---|---|
| D4029 | DNA Clean & Concentrator-100 | 25 Preps |
| D4030 | DNA Clean & Concentrator-100 | 50 Preps |
The DNA Clean & Concentrator-100 (DCC-100) is a PCR purification kit designed for the rapid desalting and purification of up to 100 μg of high quality DNA from PCR, large format restriction endonuclease digestions, or cell-free lysates. Eluted DNA is suitable for nucleotide sequencing, array analysis, PCR, nucleotide blotting, restriction endonuclease digestion procedures, as well as many other downstream applications requiring high quality DNA. The entire DNA purification/concentration procedure typically takes less than 20 minutes and can be performed using a syringe, centrifuge or vacuum source together with a microcentrifuge.
| Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
|---|---|
| Elution Volume | ≥ 150 μl |
| Equipment | Microcentrifuge and centrifuge or vacuum source. |
| Purity | Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Sample Source | DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
| Size Range | 50 bp to 23 kb |
| Yield | ≤ 100 μg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
