Description
Quickly Concentrates Up To
20 mL of Biological Sample
Rapidly concentrates 20 mL sample volumes to 0.5 mL
Provides high recoveries, typically > 90%
Low protein-binding Omega? membrane and polypropylene housing minimize losses due to non-specific binding
Versatile Omega membrane is available in a variety of MWCOs
Built-in deadstop prevents spinning to dryness
Color-coded for easy identification
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Applications
Ultrafiltration
Concentrate and desalt proteins
Exchange buffer or remove salt of chromatography eluates and gradient fractions
Recover proteins or other molecules from cell culture supernatants
Microfiltration
Remove particulate from aqueous solutions and clinical samples
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Specifications
Materials of Construction
Filter Media: Omega (modified polyethersulfone) and Supor? (polyethersulfone) membranes
Sample Reservoir, Filtrate Receiver, and Cap: Polypropylene
Paddle: Polyethylene
Effective Filtration Area
7.2 cm2
Dimensions
Diameter: 29 mm (1.2 in.)
Length: 12.0 cm (4.7 in.)
Operating Temperature Range
0 - 40 °C (32 - 104 °F)
Capacities
Maximum Sample Volume: 20 mL
Final Concentrate Volume:
450 μL (swinging bucket)
1.2 - 1.5 mL (45o angle rotor)
1.5 mL (34o angle rotor)
Filtrate Receiver Volume: 22 mL
Hold-Up Volume: 80 μL (membrane and paddle)
pH Range
1 - 14
Maximum Centrifugal Force
5,000 x g (ultrafiltration)
14,000 x g (microfiltration)
Centrifuge
Fits centrifuges that accept standard 50 mL conical end tubes
Sanitization
Provided non-sterile. May be sanitized by filtering 70% ethanol through thedevice prior to use.
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Performance
Macrosep Advance Centrifugal Devices: Reduced Spin Time
Protein solutions were processed in each of the Macrosep Advance devices. Average time (minutes) is plotted against mL of remaining product to be filtered using a swinging bucket rotor at 5,000 x g. Solutions are 3K: Protamine Sulfate, 0.1% in 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.
Macrosep Advance Centrifugal Devices: Retention Efficiency
Protein solutions were processed in each of the Macrosep Advance devices. Average percent retention using a swinging bucket rotor at 5,000 x g is displayed for each MWCO. Solutions were 3K: Protamine Sulfate, 0.1% 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.
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Type
Centrifugal Device
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Use
Ultrafiltration
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