品牌:美國 NewEast Biosciences
貨號: 26163
抗原:Synthetic peptide
規(guī)格:100 微升
產(chǎn)品全名:Anti-IDH2(R172K) Mouse Monoclonal Antibody
應(yīng)用: ELISA, WB, IF, IHC
種屬反應(yīng)性的選擇:Human, Mouse and Rat
物種/宿主:Mouse
描述: Anti-IDH2(R172K) Mouse Monoclonal Antibody
背景介紹: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas.
免疫原: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin
經(jīng)過測試的應(yīng)用: ELISA, WB, IHC
推薦稀釋比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IHC: 1: 50-1: 100 IHC: 1: 10-1: 100
濃度: 1 mg/ml
宿主: Mouse
形式: Liquid
克隆性: Monoclonal
亞型: IgG
純度: Purified from ascites
防腐劑: No
成分: PBS (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
種屬反應(yīng)性: recognizes R172K mutant, but not wild-type IDH2 of vertebrates
儲存條件: Store at -20°C. Avoid repeated freezing and thawing
蛋白質(zhì)印跡:
Western blot analysis of recombinant IDH2(R172K) and wildtype proteins. Purified His-tagged IDH2(R172K) (lane 2) and wildtype protein (lane 1) were blotted with Anti-IDH2(R172K) monoclonal antibody (Cat. #26163). 免疫熒光:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172K) antibody. HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #26163).
武漢費斯德生物科技有限公司是美國NewEast Biosciences在中國的辦事處，也是目前所有產(chǎn)品國內(nèi)經(jīng)銷處。
目前客戶使用產(chǎn)品發(fā)表超過1000谷歌學(xué)術(shù)文獻(xiàn)，其中論文分布排名前50刊物上。
NewEast Biosciences 在十二年前率先研發(fā)倆種獨特的抗體。這倆種抗體僅僅識別活性的GTP酶或者突變的Oncogene。 GTP酶涉及(1)響應(yīng)細(xì)胞表面受體**的信號轉(zhuǎn)導(dǎo)，包括跨膜受體，例如介導(dǎo)味覺、嗅覺和視覺的那些，（2）核糖體的蛋白質(zhì)生物合成，（3）調(diào)節(jié)細(xì)胞分化、增殖、分裂和運動，（4）蛋白質(zhì)通過膜的易位，（5）細(xì)胞內(nèi)囊泡的運輸，以及囊泡介導(dǎo)的分泌和攝取，通過GTP酶控制囊泡外殼組裝。Oncogene側(cè)是誘發(fā)癌癥的基因。
更多產(chǎn)品類目和技術(shù)資料可查看：武漢費斯德生物官網(wǎng)。
