3D單分子熒光成像系統(tǒng)-SAFe 360 |
SAFe 360是法國(guó)abbelight公司推出的一款基于單分子定位的顯微成像（SMLM）的新型3D單分子成像系統(tǒng)，它的DAISY技術(shù)整合了散光技術(shù)和超臨界角光技術(shù)，能夠大的提高定位精度，xyz三軸定位精度高達(dá)15nm，可以提供高清晰三維亞細(xì)胞結(jié)構(gòu)圖像，支持同時(shí)多四色成像，可以用于細(xì)胞納米三維成像，觀測(cè)高清晰亞細(xì)胞器結(jié)構(gòu)，實(shí)時(shí)研究不同的結(jié)構(gòu)功能蛋白的共定位信息，在單分子水平研究分子動(dòng)力學(xué)反應(yīng)以及細(xì)胞間的相互作用等。
3D單分子熒光成像系統(tǒng)-SAFe 360
設(shè)備參數(shù)
+ 成像模式：PALM、STORM、PAINT、smFRET 、SPT
+ 光源模式：Epi、TIRF、HILO
+ 超高分辨率：15 nm的XYZ軸分辨率
+ 超大視野：200 × 200 μm2的視野
+ 一次可同時(shí)采集1.2 μm深度圖像信息
+ 大圖像深度：10 μm
+ 實(shí)時(shí)漂移矯正
+ 多四色同時(shí)成像
+ 活細(xì)胞成像模式
| 加裝 | TIRF PALM STORM SPT smFRET ...... | 兼容 | Confocal Spinning-Desk Widefield SIM STED |
Now We See......
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| 3D線粒體結(jié)構(gòu) | 核孔復(fù)合物 |
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| 老鼠海馬神經(jīng)元 | 微管蛋白網(wǎng)絡(luò) |
配套試劑
| Smartkit | Compatibledyes |
- 10 doses per box - 200 μL per dose - 30 sec prepartion - 2 months in a fridge - 2 weeks on sample | - Atto 488, WGA-AF?488 - AF?532, CF?532, Cy3b - AF?555, AF?594, CF?555, AF?568, CF?568, Cy5, MemBriteTM 568, TMR - AF?647, CF?647, AF?680, CF?680, MemBriteTM 640, Actin-stain 670, SiR647 |
發(fā)表文獻(xiàn)列表
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[5] Mau, Adrien, et al. "Fast scanned widefield scheme provides tunable and uniform illumination for optimized SMLM on large fields of view." bioRxiv(2020).
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[10] Denis, Kevin, et al. "Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease." Nature microbiology4.6 (2019): 972.
[11] Szabo, Quentin, et al. "TADs are 3D structural units of higher-order chromosome organization in Drosophila." Science advances4.2 (2018): eaar8082.
[12] Boudjemaa, Rym, et al. "Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus." Antimicrobial agents and chemotherapy62.7 (2018): e00023-18.
[13] Culley, Sian, et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods15.4 (2018): 263.
[14] Berger, Stephen L., et al. "Localized myosin II activity regulates assembly and plasticity of the axon initial segment." Neuron97.3 (2018): 555-570.
[15] Cabriel, Clément, et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters43.2 (2018): 174-177.
[16] Bouissou, Ana?s, et al. "Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring." ACS nano11.4 (2017): 4028-4040.
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用戶單位
