品牌:美國(guó) NewEast Biosciences
貨號(hào): 26039
抗原:Synthetic peptide
規(guī)格:100 微升
產(chǎn)品全名:Anti-BRaf(V600E) Mouse Monoclonal Antibody
應(yīng)用: ELISA, WB, IF, IHC
種屬反應(yīng)性的選擇:Human, Mouse and Rat
物種/宿主:Mouse
貨號(hào): 26039
基因符號(hào): BRaf
描述: Anti-BRaf(V600E) Mouse Monoclonal Antibody
背景介紹: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers
免疫原: A synthetic peptide from the internal region of BRaf(V600E), human origin
經(jīng)過(guò)測(cè)試的應(yīng)用: ELISA, WB, IHC, IP
推薦稀釋比:
ELISA: 1: 100-1: 5000
WB: 1: 100-1: 2000
IHC: 1: 100-1: 1000
濃度: 1 mg/ml
宿主: Mouse
形式: Liquid
克隆性: Monoclonal
亞型: IgG2b
純度: Purified from ascites
防腐劑: No
成分:PBS (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
種屬反應(yīng)性: Recognizes B-Raf(V600E), but not wild type B-Raf proteins from vertebrates.
儲(chǔ)存條件: Store at -20°C. Avoid repeated freezing and thawing
蛋白印跡：
Western blot analysis of recombinant B-raf proteins with anti-BRaf(V600E) antibody.
Purified His – tagged B-Raf( V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His – tagged B-Raf( V600D ) (lane 3) , His – tagged B-Raf( V600A ) (lane 4) , His – tagged B-Raf( V600G ) (lane 5) , His – tagged B-Raf( V600K ) (lane 6), His – tagged B-Raf( V600R ) (lane 7), and His – tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039) .
免疫熒光：
Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid, pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid, pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid, pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039).
Immunohistochemistry:
B-Raf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.
Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti-BRaf (V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C. An HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as the secondary antibody. Allele-specific PCR validated to be Negative for BRAF V600E.
武漢費(fèi)斯德生物科技有限公司是美國(guó)NewEast Biosciences在中國(guó)的辦事處，也是目前國(guó)內(nèi)經(jīng)銷處。
目前客戶使用產(chǎn)品發(fā)表超過(guò)1000谷歌學(xué)術(shù)文獻(xiàn)，其中論文分布排名前50刊物上。
NewEast Biosciences 在十二年前率先研發(fā)倆種獨(dú)特的抗體。這倆種抗體僅僅識(shí)別活性的GTP酶或者突變的Oncogene。 GTP酶涉及(1)響應(yīng)細(xì)胞表面受體**的信號(hào)轉(zhuǎn)導(dǎo)，包括跨膜受體，例如介導(dǎo)味覺(jué)、嗅覺(jué)和視覺(jué)的那些，（2）核糖體的蛋白質(zhì)生物合成，（3）調(diào)節(jié)細(xì)胞分化、增殖、分裂和運(yùn)動(dòng)，（4）蛋白質(zhì)通過(guò)膜的易位，（5）細(xì)胞內(nèi)囊泡的運(yùn)輸，以及囊泡介導(dǎo)的分泌和攝取，通過(guò)GTP酶控制囊泡外殼組裝。Oncogene側(cè)是誘發(fā)癌癥的基因。
更多產(chǎn)品類目和技術(shù)資料可查看：武漢費(fèi)斯德生物科技有限公司官網(wǎng)。
